In this paper, the connotation and characteristics of university culture are specified in terms of spiritual culture, institution culture, material and environmental culture, and behavior culture. Based on the defined connotation and characteristics, the components of university culture are analyzed and summed up, the simulated flow diagram of system dynamics of the components is established with university culture as an example, the effect of university spiritual culture construction is analyzed with simulation, and the key influential factors of university spiritual culture construction are clarified.